Fig. 1.

Effect of pH on the elastase recovery and purification factor (PF). Enzyme partitioning at certain pH was done in systems containing 20% (w/w) PEG 2 000 and 13.2% (w/w) KH₂PO₄-K₂HPO₄. Three replicates were made to estimate experimental error and where a, b, c over the columns stand for the significant difference (of recovery) by Duncan’s multiple range test (P=0.05)