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Experimental design and results of path of steepest ascent
| No. | X1 (%) | X2 (%) | Y (%) |
| 10 | 20.8 | 14.2 | 77.6 |
| 11 | 21.6 | 13.4 | 80.9 |
| 12 | 22.4 | 12.6 | 82.3 |
| 13 | 23.2 | 11.8 | 85.7 |
| 14 | 24.0 | 11.0 | 81.8 |
| 15 | 24.8 | 10.2 | 78.0 |
X 1: Concentration of PEG 2 000, % (w/w); X 2: Concentration of KH2PO4-K2HPO4, % (w/w); Y: Recovery of elastase (%)
