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. 2006 Mar 6;34(5):1305–1316. doi: 10.1093/nar/gkl004

Figure 3.

Figure 3

(a) ESI-FTMS of 3.5 µM SL2 in 100 mM ammonium acetate (pH 7.5) and 10% volume 2-propanol. In the absence of ligand, SL2 provided an experimental mass of 6128.89 Da (6128.86 Da calculated from sequence). (b) ESI-FTMS of 3.5 µM SL2 in the presence of 1-fold equimolar mixture of mitoxantrone (MT), neomycin B (NB) and vancomycin (see Materials and Methods). The 1:1 complex MT•SL2 provided a mass of 6573.11 Da (6573.06 Da calculated), while NB•SL2 provided 6473.20 Da (6473.17 Da calculated). Unbound vancomycin was observed with a mass of 1407.45 Da (1407.43 Da calculated). (c) 3.5 µM SL2 with 5-fold equimolar mixture of MT, NB and vancomycin. Higher order stoichiometries were observed for MT (e.g. 2MT•SL2), resulting in increments of 444.20 Da.