. Author manuscript; available in PMC: 2006 Mar 7.

Published in final edited form as: J Biol Chem. 2004 May 20;279(30):31171–31176. doi: 10.1074/jbc.M403701200

Table I.

β-galactosidase activities from E. coli strain JT31 containing the lacZ reporter construct pUTC03 and various putA gene constructs

PutA Protein	β-galactosidase activity (U OD⁻¹)^a	Percent Activity
none	17.0 ± 0.8	100
PutA	3.3 ± 1.4	19
PutA669	0.18 ± 0.01	1
PutA90	0.27 ± 0.04	< 2
PutA47	0.17 ± 0.02	1
PutAΔ85	12.9 ± 1.5	76

Optical density was measured at 600 nm prior to cell harvesting. E. coli strain JT31 has no β-galactosidase activity.