Fig. 5. (A) The top two panels (0 day and 5 day) represent silver-stained 2-D gels of thylakoid membranes from wild-type cells before and after 5 days of growth in Fe-deficient (0 µM Fe) medium, respectively. The labeled spots have been identified previously as follows: spots 1 and 2 correspond to the inner LHCI subunits Lhca1 and presumably Lhca4, respectively, while the labeled spots correspond to isoforms of Lhca3. (B) Immunodetection of LHCI subunits in thylakoid membranes separated by 2-DE as shown in (A), using an anti-serum (18.1; Bassi et al., 1992) which recognizes epitopes that are common to most of the LHCI subunits.