Fig. 7. Immunoblots showing the susceptibility of Lhca3 and components of the major trimeric complex of LHCII in wild-type thylakoids to proteolysis when mixed with thylakoids from Fe-deficient or Fe- and Cu-deficient cells. Chloroplast ATPase accumulation (CF₁) is shown to demonstrate equal loading and specificity of the proteolytic activity. Incubation of the thylakoids at 4°C or 37°C, with or without protease inhibitor is indicated (protease inhibitor cocktail set II; Calbiochem).