Fig. 1. Deletion analysis of the yeast mitochondrial LeuRS splicing factor. (A) Homology model of the yeast mitochondrial LeuRS. The CP1 domain (red) is linked by two β-strand linkers, shown in light (N-terminal) and dark (C-terminal) blue. The C-terminal flanking region has a Zn²⁺-binding motif called Zn-2 (purple). An α-helical hairpin (yellow) links the N-terminal flanking region denoted as Zn1-like (green). Space-filling regions represent splicing-sensitive mutation sites. (B) Cartoon of the LeuRS CP1 primary sequence. Black arrows marked by ‘F’ and ‘R’, respectively, show forward and reverse primer sites used to amplify the gene regions encoding LeuRS fragments. Solid rectangles are Zn²⁺-like or Zn²⁺-binding motifs. (C) Schematic and sizes of LeuRS fragments that contained CP1.