Fig. 2. Rescue of a splicing-deficient mutation by protein fragments that contained the LeuRS CP1 domain. (A) The yeast mitochondrial LeuRS deletion strain QBY320, which contains a splicing-deficient M.tuberculosis LeuRS mutant W286C [msl1Δ::HIS3 ρ^–(pSBR-Mtb-W286C)] (Houman et al., 2000), was transformed with plasmids expressing different sub-genes for LeuRS fragments that contained CP1. Transformants were grown on glycerol (upper plate) and glucose (lower plate) medium (Houman et al., 2000). A positive control bearing the wild-type M. tuberculosis LeuRS (top left) grows on glycerol medium, while the W286C splicing mutant (top right) fails to complement the null strain, as described previously (Houman et al., 2000). Each of the six separate LeuRS CP1-containing protein fragments rescues the mutant W286C LeuRS. (B) Transformants included the wild-type (top left) and Q273A mutant (top right) yeast mitochondrial LeuRSs. The M.tuberculosis W286C mutant LeuRS is complemented by the wild-type LeuRS CP1-containing fragment (bottom left), but not the corresponding Q273A mutant (bottom right).