Fig. 8. The mitochondrial Ca²⁺ uptake inhibitor ruthenium red reduces the ability of I_CRAC to activate in weak intracellular Ca²⁺ buffer following store depletion with thapsigargin. (A) The time course of activation of I_CRAC is compared between a control cell (filled circles; dialysed with thapsigargin and 0.1 mM EGTA) and one in which ruthenium red (100 µM) had been added to this pipette solution (open circles). (B) Aggregate data for the two conditions are summarized [nine cells for control and eight for ruthenium red (RuR)].