Fig. 6. Enhanced depolymerization of microtubules in HDAC6-overexpressing cells. NIH 3T3 cells transfected with an HA-HDAC6 expression vector were pretreated with ethanol (A), 100 nM TSA (B) and 3 µM TSA (C) for 6 h and then exposed to demecolcin for 10 min. Microtubules were stained with the anti-α-tubulin antibody and the HDAC6-transfected cells were visualized with the anti-HA antibody.