Fig. 3. Northern analysis of plants transformed with FLAG-tagged PNPase. (A) Schematic diagram of the location of the probes used for northern hybridizations within the rbcL and psbA mRNA (black bars). (B and D) Northern hybridizations with rbcL- and psbA-specific probes corresponding to the respective coding regions (CDS). The labeling of the investigated lines corresponds to Figure 2D. (C and E) Identical membranes were analyzed with probes specific for the unprocessed 3′ trailer (3′ UTR) of rbcL and psbA, respectively. (F) Control hybridization with a probe for the nuclear-encoded 18S rRNA. The numbers indicate the sizes of the detected RNA species (in kb).