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. 2001 Aug;13(8):1791–1802. doi: 10.1105/TPC.010020

Figure 2.

Figure 2.

RNA Gel Blot and RT-PCR Analyses of the Expression of GAI or gai Transcripts in Transgenic Rice.

(A) UV fluorescence of ethidium bromide–stained gel containing RNA from plants (nontransgenic control and 1, G36-1; 2, G36-2; 3, G38-4; 4, G38-17; 5, G39-3; 6, G39-8; 7, G41-22; 8, G41-26; 9, g51-1; 10, g51-6; 11, g58-1; 12, g58-2; and 13, g59-1) used for the hybridization shown in (B).

(B) RNA gel blot analysis of RNA shown in (A) using a radiolabeled GAI ORF as probe.

(C) RT-PCR analysis of GAI/gai transcript levels in RNA from R1 plants. RT-PCR amplification was for 25 cycles using the gai-1 and gai-2 primers (see Methods and Figure 1A).

(D) RT-PCR control showing that actin transcripts are detected consistently in all of the samples used for the GAI/gai RT-PCR experiment shown in (C).