Figure 5.

Rice 20-Oxidase Transcripts Are Upregulated by Expression of GAI or gai.

(A) RNA gel blot analysis of 20-oxidase transcripts in GA-treated Basmati rice seedlings. One-week-old seedlings were sprayed with 100 μM GA₃. Leaf RNA samples were prepared 8, 16, and 24 hr after treatment.

(B) The relationship between 20-oxidase and gai/GAI transcripts in transgenic rice plants. Top, UV fluorescence of an ethidium bromide–stained gel containing RNA prepared from plants G43-5 (a), G39-8 (b), g51-1 (c), g48-3 (d), and a control, untransformed Basmati rice plant. Beneath the UV fluorescence is an RNA gel blot hybridization from this gel, using a radiolabeled GAI ORF probe. Beneath this is shown a further hybridization, using a radiolabeled 20-oxidase probe.