Figure 2.

RNA Gel Blot Analysis of Fruit RNA in Normal and Transgenic Lines.

Total RNA was extracted from fruit of two different transformed lines at different stages of ripening: mature green (MG), breaker (B), and five (B+5), ten (B+10), or twenty (B+20) days after breaker. It was then separated on a 1% agarose-formaldehyde gel and transferred onto a nylon membrane. The blot was hybridized with either a sense-specific or an antisense-specific ³²P-labeled LeRab11a riboprobe. Membranes were washed twice in 2 × SSC and 0.1% SDS at 65°C for 5 min and then twice in 0.1 × SSC and 0.1% SDS for 15 min at 65°C. The stained rRNA bands are shown under each lane to show equivalence of loading.

(A) Transformed lines T1 (pBINDR) and T5 (pBINTR) and wild-type (WT) control tomato with an antisense-specific riboprobe.

(B) Transformed lines T1 and T5 and wild-type control tomato with a sense-specific riboprobe.