Table 2.

DNA Gel Blot Analysis of AIF Probes That Showed AFLP Band Disappearance in the F1 Hybrid and/or in the Allotetraploid Plants of Cross B (S^lS^l × AA)

Probe	Primer Pairs	Fragment Size (bp)	Origin of Fragment	Eliminationa	Copy Number	Sequence Similarityb
AIF1	E+AAC/M+CAG	420	urartu	+	Low	None
AIF2	E+AAC/M+CAG	202	urartu	+	Low	None
AIF3	E+AAC/M+CAG	150	urartu	+	Low	None
AIF4	E+AAC/M+CAG	80	urartu	ND	Low	None
AIF5	E+AGG/M+CTT	530	longissima	ND	High	None
AIF6	E+AGG/M+CTT	510	urartu	+	Low	None
AIF7	E+AGG/M+CTT	450	urartu	+	Low	None
AIF8	E+AAC/M+CTT	170	longissima	+	Low	None
AIF9	E+AAC/M+CTT	165	longissima	+	Low	None

^aElimination in F1 and/or the allotetraploid was validated (+) or could not be determined (ND). AIF1 to AIF7 were eliminated in the allotetraploid only, and AIF8 and AIF9 were eliminated in both F1 and the allotetraploid.

^bSimilarity was considered significant for e values ≤ 0.005.