Table 5.

DNA Gel Blot Analysis of the Fragments That Showed Methylation Alteration in the F1 Hybrid of the Cross Ae. sharonensis (S^lS^l) and T. monococcum ssp aegilopoides (A^mA^m) and in the Derived Allotetraploid

				Methylation Alterationb
Probe	Primer (HM+4/E+3)a	Fragment Size (bp)	Origin of the Fragment	F1	Amphiploid	Copy Number	Sequence Similarityc
MIF1	E+AAG	210	sharonensis	+	+	High	Barley retrotransposon
MIF2	E+AAG	260	aegilopoides	+	+	Low	None
MIF3	E+AGG	100	sharonensis	+	+	High	Barley retrotransposon
MIF4	E+AGG	150	aegilopoides	+	+	High	Ceratitis capitata unknown   repetitive DNA fragment
MIF5	E+ACA	134	aegilopoides	+	+	High	None
MIF6	E+AAG	350	aegilopoides	−	+	Low	None
MIF7	E+ACT	110	aegilopoides	−	+	High	None
MIF8	E+ACT	240	sharonensis	−	+	Low	None
MIF9	E+ACA	270	aegilopoides	−	+	High	None
MIF10	E+ACC	120	aegilopoides	−	+	Low	None
MIF11	E+ACC	405	aegilopoides	−	+	Low	None
MIF12	E+ACC	275	aegilopoides	−	+	High	None

^aThe selective primer HM+4 (5′-CATGAGTCCTGCTCGGTCAA [HM+TCCA]) was used in combination with each of the four E+3 primers listed. The core sequence of EcoRI is identical to that used in the AFLP protocol by Vos et al. (1995).

^b+, methylation alteration; −, no alteration.

^cSimilarity was considered significant for e values ≤ 0.005.