Table 1.

Effect of Glucose and Scytalone on the C. lagenarium clapex6 Mutant

		Appressorium		Lesion Formationd (%)
Strain	Compounda	Melanizationb	Sizec (μm)
104-T	Water	+++	6.91 ± 0.39	99.62
DPE1	Water	−/+	6.08 ± 0.56	1.01
DPE1	Glucose	++	6.75 ± 0.38	21.20
DPE1	Scytalone	++	6.13 ± 0.41	1.41

^aConidia were incubated in water, 1 mM glucose solution, or 1 mM scytalone solution.

^bAppressoria of the clapex6 mutant were faintly melanized (−/+). Addition of glucose or scytalone restored melanization of the clapex6 mutant (++), although the degree of melanization did not reach the wild-type level (+++).

^cDiameters of appressoria were measured with a ×100 objective. Each value represents the average of >100 appressoria.

^dConidial suspension was spotted on detached cucumber leaves and incubated for 7 days, and the number of lesions was counted. The percentage of lesion formation was based on the total number (>200) of spotted conidial suspensions.