Figure 1.

Map-Based Cloning and Gene Structure of EMF1.

(A) Summary of the physical and genetic positions of EMF1 on chromosome (Chr) V. The top horizontal line represents the markers used to fine map the EMF1 locus between 9G2-R and 18G5-R on chromosome V. Values indicate the number of recombinant plants identified between the EMF1 locus and a given RFLP marker (circles). The bottom horizontal line shows the organization of the four genes deduced from the sequence of the CD82 clone: ORF-X (a putative ORF), the GLOxidase-like gene, EMF1, and ASP3 (aspartate aminotransferase3).

(B) Structure of the EMF1 gene and positions of the three mutations in the mutant alleles, emf1-1, emf1-2, and emf1-3. Black boxes indicate exons, and lines between the boxes indicate introns.

(C) Allele-specific RFLPs created by the emf1-1 and emf1-2 mutations. The positions of primers and enzyme restriction sites for each genotype, wild type (WT) and mutants (emf1-1 and emf1-2), are depicted in the schemes. Below, the left and right panels show the allele-specific RFLP analysis associated with the emf1-1 and emf1-2 alleles, respectively. Molecular mass markers are identified to the right of each gel.