Figure 3.

Expression of EMF1 RNA in Wild-Type Arabidopsis.

(A) Autoradiographic determination of the relative amount of EMF1 RNA from a blot containing 1 μg of poly(A)⁺ RNA isolated from different tissues. The RNA gel blot was hybridized with an EMF1 radioactive probe and, after stripping, with a GAPc probe as a loading control. The numbers below the gels indicate the relative amounts of EMF1 RNA after standardization using the GAPc signal as a reference. Tissue samples were from roots of 2-week-old plants on agar plates; rosette leaves of 3- to 4-week-old plants in soil under short-day conditions; stems, cauline leaves, and flower clusters from an inflorescence shoot apex with developing buds and open flowers.

(B) An autoradiograph from semiquantitative RT-PCR analysis of the EMF1 level in wild-type plants grown under short-day conditions. Total RNAs were isolated from seedlings at the times indicated. RT-PCR products were amplified with EMF1 primers and GAPc primers and hybridized with an EMF1 probe (top) or a GAPc probe (bottom).