Table 1.
Complementation of emf1 Mutant Alleles by the SpeI–Asp718 Genomic DNA
Number of T2 plantsb
|
||||||
---|---|---|---|---|---|---|
T1 Line | Mutant Allelea |
KmR WT | KmS WT | KmR Mutant |
KmS Mutant |
Total |
1-4 | emf1-1 | 44 | 10 | 0 | 2 | 56 |
3-12 | emf1-1 | 45 | 15 | 0 | 5 | 65 |
3-11 | emf1-1 | 47 | 0 | 0 | 9 | 56 |
3-10 | emf1-1 | 51 | 3 | 0 | 0 | 54 |
Total | 187 | 28 | 0 | 16 | 231 | |
1-2 | emf1-2 | 107 | 32 | 0 | 11 | 150 |
2-2 | emf1-2 | 134 | 0 | 0 | 8 | 142 |
7-2 | emf1-2 | 110 | 0 | 0 | 36 | 146 |
8-2 | emf1-2 | 128 | 0 | 0 | 18 | 146 |
9-1 | emf1-2 | 81 | 11 | 0 | 8 | 100 |
9-2 | emf1-2 | 141 | 45 | 0 | 14 | 200 |
Total | 701 | 88 | 0 | 95 | 884 |
a Mutant alleles of T1 transgenic plants were confirmed by the allele-specific RFLP or by the occurrence of KmS emf1 mutants in T2 populations.
b KmR, kanamycin-resistant; KmS, kanamycin-sensitive; WT, wild type.