Figure 5.

Change in the Levels of Transcripts for NtmybA1, A2, and B during the Cell Cycle.

(A) RNA gel blot analysis of NtmybA1, A2, and B. Tobacco BY2 cells were synchronized by aphidicolin treatment. Cells were collected at 1-hr intervals after release from aphidicolin block. Gene-specific probes for NtmybA1, A2, and B were used for hybridization. To detect Nicta;CycB1;3 (CycB) mRNA, full length cDNA was used as a probe. Ethidium bromide staining of the rRNA is shown as well.

(B) Change in mitotic index during synchronous cultures. The efficiency of cell synchronization was checked by measuring the mitotic index at 1-hr intervals.