Figure 5.

Ethylene Regulates the Number of Root Hairs Activated for Calcium Spiking in Response to Nod Factor.

(A) Wild-type plants (WT) were grown on either 0.1 μM AVG or 10 μM ACC, and skl plants were grown on 10 μM ACC. Data points represent the percentage of root hairs that were induced for calcium spiking 60 min after the addition of 10 pM Nod factor.

(B) Wild-type plants (WT) were grown either on medium alone or on 0.1 μM AVG or 10 μM ACC, and skl plants were grown in the absence of either AVG or ACC. The concentration of Nod factor was increased 10-fold every 30 min, starting at 1 fM and ending at 1 nM. The number of root hairs induced for calcium spiking at each concentration was assessed.