Figure 4.

The effects of primer concentrations for the FV Bi-PASA reaction. WT/M genomic DNA was used for all reactions. Lanes 1–6, 7–12, 13–18, and 19–24 show the effects of varying only the P, Q, B11, and A11 primers, respectively. Lanes 25–30 show the effects of varying all four primers at once. The primers used and the 1× concentrations were determined by the optimal conditions used in Fig. 3B. (M) Size standard (120 ng of φX174/HaeIII).