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. 2001 Oct;13(10):2191–2210. doi: 10.1105/tpc.010192

Figure 3.

Figure 3.

Level of JA Is Decreased in the dad1 Inflorescence.

(A) and (B) Rescuing of the dad1 mutant by application of MeJA. (A) A dad1 flower opened at 2 days after application. Dehisced anthers and functional pollen grains attached to the stigma are observed. (B) Side view of the dad1 inflorescence at 2 days after application, in which there is no accumulation of unopened flower buds.

(C) to (E) Rescuing of the dad1 mutant by application of LA. (C) A dad1 flower opened at 2 days after application. Dehisced anthers and functional pollen grains attached to the stigma are observed. (D) A dehisced dad1 anther at 2 days after application. (E) A dad1 inflorescence at 7 days after application. Developing seed pods (arrows) are observed.

(F) A dad1 inflorescence at 7 days after application of linoleic acid, which cannot be converted to JA.

(G) Jasmonate levels in flower bud clusters of the wild type (WT) and the dad1 mutant. JA and MeJA were isolated, purified, and measured by gas chromatography–mass spectrometry (see Methods). The data represent the sum of both compounds. FW, fresh weight.