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DNA Gel Blot Analysis of Ntcel2, Ntcel7, and Ntcel8 Genes in Tobacco.
Genomic DNA (5 μg) was digested with BamHI (B), EcoRI (E), and HindIII (H), electrophoresed on a 0.7% agarose gel, blotted to nylon membranes, and probed with a 1-kb fragment spanning the conserved amino acid domains CWERPED and YINAPL of Ntcel2, Ntcel7, and Ntcel8. Blots were hybridized in 5 × SSC at 65°C and washed twice in 0.5 × SSC at 68°C and twice in 0.1 × SSC at 68°C. L, 1-kb DNA ladder (Gibco BRL).
