Figure 6.

In Situ Hybridization of Ntcel7, Ntcel8, and Ntcel2 mRNA in RKN-Infected Tobacco Roots.

(A) and (B) Longitudinal serial sections through a RKN-induced gall on tobacco roots 12 to 14 days after infection and hybridized with a tobacco Ntcel7 sense (A) or antisense (B) DIG-labeled riboprobe.

(C) Longitudinal section through a RKN-induced gall on tobacco roots 7 to 9 days after infection and hybridized with a tobacco Ntcel7 antisense DIG-labeled riboprobe.

(D) to (F) Longitudinal sections through tobacco roots infected with RKN and hybridized with a tobacco Ntcel8 antisense DIG-labeled riboprobe at 7 to 9 days after infection. (F) is a higher magnification of (E).

(G) and (H) Longitudinal serial sections through a tobacco gall induced by RKN 12 to 14 days after infection and hybridized with a tobacco Ntcel2 sense (G) or antisense (H) DIG-labeled riboprobe.

GC, giant cells; LR, lateral root; N, nematode. Bars = 50 μm.