Figure 7.

In Vitro Nucleic Acid Binding by HUA1.

The full-length HUA1 protein (HUA1FL), an N-terminally truncated version that contains all six zinc fingers (HUA1CT), and an unrelated protein (APA1) were expressed as His-tagged proteins, purified, and incubated with various nucleic acids attached to agarose or cellulose beads. HUA1FL binds single-stranded DNA (ssDNA), poly rA, poly rG, and poly rU at 0.1 M NaCl and poly rG and poly rU at 0.25 M NaCl. HUA1CT binds poly rG under both conditions. APA1 does not bind any nucleic acid under these conditions. Ten percent of the input proteins is shown in the right lane. dsDNA, double-stranded DNA.