Figure 3.

Amino Acid Sequence Alignment of Members of the Maize γ-Zein Protein Family.

(A) The polypeptides correspond to alleles of the 16-, 27-, and 50-kD γ-zeins and the 15-kD β-zein in the B73 inbred line (for GenBank accession numbers and abbreviations, see Table 1). Conserved Cys residues are indicated with asterisks. Identical residues are shown in boldface, and similar amino acids and conservative changes are shaded. Underlined residues indicate peptide sequences that were either expressed in bacteria or synthesized chemically and used to develop monospecific antibodies (see Methods).

(B) Dendrogram of the alignment shown in (A). The bootstrapping values of all internal branches exceeded 95% (data not shown).