Figure 5.

Amino Acid Sequence Alignment of the 18-kD α-Globulin and Related Protein Sequences.

(A) Conserved Cys residues are indicated with asterisks. Identical residues are shown in boldface, and similar amino acids and conservative changes are shaded. The “Trp box” in wheat puroindolin and a similar Trp-rich sequence in the maize 18-kD α-globulin are underlined. The unique wheat HMW glutenin repeat domain between residues 217 and 616 was omitted for brevity. GenBank accession numbers are as follows: maize α-globulin, AF371278; wheat HMW glutenin, P08488; rice α-globulin, D50643; and wheat puroindolin b, X69912.

(B) Dendrogram of the alignment shown in (A). The bootstrapping values of all internal branches exceeded 95% (data not shown).