Figure 7.

Detection of mRNAs in Maize Endosperm by in situ Hybridization of Antisense Probes.

Longitudinal median sections of maize kernels at 10 ([A] to [D]), 15 ([E] to [H]), 20 ([I] to [L]), and 25 DAP ([M] to [P]) were hybridized with antisense RNA probes of 22-kD α-zein ([A], [E], [I], and [M]), 19-kD B1 α-zein ([B], [F], [J], and [N]), 27-kD γ-zein ([C], [G], [K], and [O]), and 16-kD γ-zein ([D], [H], [L], and [P]). Most sections for a particular developmental stage were derived serially from one or two kernels. Embryos are found at the lower right corners of the sections. Bars = 1 mm.