Figure 10.

Analysis of STAT5 activation by electrophoretic mobility shift assay in KIM-2 cells stimulated with prolactin. Nuclear extracts were prepared from confluent KIM-2 cultures stimulated with prolactin, in the absence of EGF, for the time periods indicated. The track labelled Mamm is an extract from a day 2 lactation mouse mammary gland. Uninduced control cells were maintained in growth medium. Extracts (4 μg protein) were incubated with a radioactively labelled DNA probe containing the highest affinity ovine BLG STAT5 binding site [34] and complexes resolved on a nondenaturing 6% acrylamide gel. A representative of more than three experiments is shown.