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. 2000 May 24;2(4):311–320. doi: 10.1186/bcr73

Figure 5.

Figure 5

Transient expression of FGFR2 clones isolated from SUM-52PE. (a) Whole-cell lysates from 293 cells that had been transiently transfected with individual FGFR2 clones were immunoblotted with an anti-FGFR2 antibody (lanes 2-5). Whole-cell lysate from the SUM-52PE breast cancer cell line was loaded in lane 1 as a positive control, whereas whole-cell lysates from a mock transfection of 293 cells was loaded in lane six as a negative control. (b) Whole-cell lysates from 293 cells that were transiently transfected with individual FGFR2-C3 clones containing a Flag sequence were immunoblotted with an anti-Flag antibody (lanes 1, 2, and 4). Whole-cell lysates from a mock transfection of 293 cells were loaded in lane 3 as a negative control.