Fig 1.

Establishment of L-shaped tRNA through noncovalent association of the two domains. (a Upper) The tRNA cloverleaf and its rearrangement into an L-shaped structure with tertiary interaction common to all tRNAs shown with dashed lines. (a Lower) Noncovalent assembly of RNA domains to make a tRNA-like structure. Trbp111 dimer is shown as gray spheres. Base pairing between loops is shown with dashed lines. (b) Sequence of the RNA minihelix and anticodon SBL used in the experiments. The 7-nt loop and the first two base pairs of the closing stem from RNA I and RNA II (12) are highlighted in pale gray.