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. 2002 Dec 12;99(26):16631–16635. doi: 10.1073/pnas.262667999

Fig 2.

Fig 2.

Formation of the RNA dimer complex through complementary loop–loop interactions. A gel retardation assay was carried out at pH 7, 4°C, with 5′-32P-labeled minihelix 35-mer (≈1 nM) in which unlabeled anticodon SBL was added in increasing concentrations from 0 to 15.5 μM. The shifted band was quantified and plotted on a logarithmic scale to obtain the binding profile shown on the left. The corresponding Hill plot is shown on the right.