Fig 3.

Mutation in the loop sequence disrupts complex formation. (a) Sequence of the loop used for minihelices (mut-3) and (mut-5). The potential base pairing interactions are shown with dotted lines. (b) Gel retardation assay using mutant forms of minihelix. Each 5′-³²P-labeled minihelix (≈900 nM) was incubated with unlabeled anticodon SBL for 30 min at room temperature before loading on the gel. (c) Competition assay using unlabeled minihelices. Each unlabeled minihelix was added in increasing concentrations from 10 nM to 10 μM into the mixture of 5′-³²P minihelix (≈1 nM) and unlabeled anticodon SBL (1.6 μM).