Fig 6.

(a) Mutation in the loop sequence disrupts ternary complex formation. 5′-³²P-labeled anticodon SBL (≈850 nM) was incubated with Trbp111 (37 μM) and unlabeled minihelix and minihelix (mut-3) in increasing concentrations from 100 nM to 100 μM. (b) Competition assay using unlabeled minihelices. 5′-³²P minihelix was incubated with unlabeled anticodon SBL (20 μM) and Trbp111 (1.9 μM) to which unlabeled minihelix or minihelix (mut-3) was added in increasing concentrations from 100 nM to 100 μM.