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Effect of quercetin on TNF-α gene expression as quantitated by real-time Q-PCR. PBMCs were cultured for 24, 48, and 72 h with quercetin at 1, 5, 10, 25, and 50 μM, and TNF-α gene expression was quantitated. Data represented are means ± SD of results from three separate experiments. Statistical significance was determined by Student's t test. TAI, transcript accumulation index.
