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FACS analyses to determine the cell population in PBMCs producing TNF-α. (a) Isotype control; (b) TNF-α and CD4+ cells, control; (c) TNF-α and CD4+, quercetin (50 μM, 48 h); (d) TNF-α and CD14+ cells, control; (e) TNF-α and CD14+ cells, quercetin (50 μM, 48 h); (f) graph showing the mean percentage ± SD of positive cells. Statistical significance was determined by Student's t test (n = 3). con, control.
