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Reactivity of identified full-length recombinant proteins to pooled LL/BL sera by Western blot. Lanes 1 and 4, ML2331; lanes 2 and 5, ML0405; lanes 3 and 6, ML2055. Lanes 1 to 3 developed with anti-His monoclonal antibody (BD Biosciences). Lanes 4 to 6 developed with pooled LL patient sera used for expression cloning (n = 5). Positions of molecular mass markers are indicated in kilodaltons. The experiment was repeated three times, and a typical representation of the results is shown.
