IgM and IgG reactivity of six LL index cases, household contacts, and normal sera to (A) NDOHSA, (B) ML0405, (C) ML2331, and (D) ML2055, measured by ELISA. Serum dilution was 1:200. Number of index case serum samples was 6, the number of household contact (HC) serum samples was 28, and the number of NEC (normal) serum samples was 5. Each index case had a minimum of four household contacts included in this study. An arbitrary cutoff for reactivity of an OD of 0.2 is indicated by a horizontal line.