TABLE 1.
Antigens of M. leprae identified by expression cloning using pooled LL/BL patient serad
| Antigen | No. of clonesa | Sizes (kDa) | TB homologue (% identity) | Description | Mean ELISA OD450 ± SDb
|
|
|---|---|---|---|---|---|---|
| LL/BL | NEC | |||||
| ML0050/ML0049 | 2 | 10, 9 | Rv3874 (40.0) | Secreted; CFP-10, ESAT-6 | 0.52 ± 0.87c | 0.06 ± 0.01 |
| Rv3875 (36.3) | ||||||
| ML0091 | 4 | 23.7 | Rv3810 (52.7) | Potentially secreted | ND | ND |
| ML0317 | 2 | 56.9 | Rv0440 (94.8) | GroEL2, 60-kDa chaperonin 2 | ND | ND |
| ML0405 | 2 | 40.7 | Rv3616c (62.7) | Previously unknown | 2.31 ± 0.45 | 0.13 ± 0.02 |
| ML0568 | 5 | 18.3 | Rv1435c (38.9) | Previously unknown | 0.34 ± 0.22 | 0.13 ± 0.04 |
| ML1213 | 3 | 80.1 | Rv1565c (76.5) | Previously unknown | 0.51 ± 0.21 | 0.23 ± 0.04 |
| ML2028 | 3 | 34.8 | Rv1886c (88) | Secreted; Ag85b | 2.29 ± 1.26 | 0.52 ± 0.21 |
| ML2055 | 6 | 29.5 | Rv1860 (66.8) | Membrane protein | 1.87 ± 1.19 | 0.19 ± 0.05 |
| ML2655 | 2 | 36.3 | Rv0129 (82.0) | Secreted; Ag85c | 2.82 ± 0.81 | 1.88 ± 0.28 |
| ML0097 | 1 | 35.4 | Rv3804c (82.0) | Secreted; Ag85a | ND | ND |
| ML1812 | 1 | 50.5 | Rv1477 (78.8) | Previously unknown | ND | ND |
| ML2331 | 2 | 26.5 | Rv3717 (82.0) | Previously unknown | 2.09 ± 1.07 | 0.11 ± 0.06 |
| ML2496 | 3 | 24.2 | Rv0351 (68.6) | DnaK, 70-kDa heat shock protein | ND | ND |
a
Number of different clones identified in the screen encoding the full or partial sequence of the same antigen.
b
OD450 ± standard deviation in ELISA developed with protein A at a serum dilution of 1:200 (minimum of n = 6 for each measurement). ND, not done.
c
ELISA results with ML0050-ML0049 fusion protein.
d
n = 5.