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. Author manuscript; available in PMC: 2006 Mar 8.
Published in final edited form as: J Immunol. 2005 May 15;174(10):5987–5993. doi: 10.4049/jimmunol.174.10.5987

FIGURE 5.

FIGURE 5

TGF-β1 does not affect FcεRI mRNA expression. A, BMMC were cultured in IL-3 plus vehicle or TGF-β1 (5 ng/ml) for the indicated times. Down-regulation of surface FcεRI expression was confirmed by flow cytometry on day 3 of culture (not shown). The expression of FcεRI subunits and control mRNAs was determined by RPA using total RNA. B, RPA results were quantified by phosphorimaging, using L32 and GAPDH housekeeping gene expression as loading controls. The expression of each FcεRI subunit mRNA relative to the sum of housekeeping gene expression in each lane is shown. Data are the mean and SE of four separate samples.