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. 2006 Mar 7;3(4):e100. doi: 10.1371/journal.pmed.0030100

Figure 2. MMP Expression in Kidneys of Patients with XAS and in Normal Human Kidneys.

Figure 2

(A–F) Frozen sections of normal human kidneys and kidneys from patients with ESRD due to XAS were stained with antibodies to MMP-2, MMP-3, and MMP-9 (FITC-green), plus laminin (Rhodamine red). Representative stainings of samples obtained from a kidney designated for transplantation or from normal portions of resected kidneys with renal cell carcinoma are displayed. (A–C) Control. (D–F) Kidneys ( n = 2) obtained from male patients with XAS.

(G–I) The bar graphs summarize the quantification of MMP-2–staining (G), MMP-3–staining (H), and MMP-9–staining (I). The left bars summarize the evaluation of MMP-staining in the glomeruli; the right bars summarize the MMP-staining in the total kidney cortex in each panel. *** p < 0.001 versus control; ** p < 0.005 versus control; * p < 0.01 versus control.

(J–L) Total protein was isolated from normal human kidneys and from two kidneys obtained from two different patients with ESRD due to XAS. Protein was analyzed by SDS-PAGE and immunoblot using specific antibodies to MMP-2 (J), MMP-3 (K), and MMP-9 (L). The lower blots in each panel display the actin control blot to control for equal protein loading.