Fig 3.

Identification of SCAP mutation in SRD-5 cells. (A) Nucleotide and deduced amino acid sequences of wild-type SCAP transcript (Wild Type) and mutant SCAP transcript (Mutant) from SRD-5 cells. The mutated nucleotide and resulting predicted amino acid are indicated by black boxes. (B) Location of these mutations in sterol-sensing domain (shaded) of hamster SCAP. The newly identified mutation, L315F, and the two reported mutations, D443N (10) and Y298C (8), are denoted by arrows. Residues conserved in SCAP proteins from human, hamster, C. elegans, and D. melanogaster are shown in red. Sequences of SCAP proteins from human, hamster, C. elegans, and D. melanogaster were aligned by using the CLUSTALW program (DNASTAR, Madison, WI). GenBank accession nos. are D83782, U67060, 247808, and U38238, respectively.