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SDS/PAGE analysis of purified PPK2. Samples were precipitated with 0.015% deoxycholate-5% trichloroacetic acid (18). Proteins were visualized by Coomassie blue staining. Samples of two different purification operations (A and B), both with nearly the same specific activity of 5.5 × 106 units/mg, were applied as indicated. M, protein molecular mass markers (Precision Protein Standards, Bio-Rad).
