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Chain lengths of poly P synthesized by PPKs. PPK2 of P. aeruginosa (Pa; 140 ng); PPK1 of P. aeruginosa (Pa; 120 ng); PPK1 of E. coli (Ec; 29 ng). After 45 min at 37°C, the products were separated by PAGE (20% gel) containing 7 M urea and visualized by PhosphorImager (Molecular Dynamics); the positions to which [γ-32P]ATP and [γ-32P]GTP migrated on the gel are indicated.
