Fig. 4.

Nucleotide (ppk2) and the predicted amino acid (PPK2) sequences of P. aeruginosa. The nucleotide sequence (5′ to 3′) of the noncoding strand starts at nucleotide 1 and terminates at 1074. A possible Shine–Delgarno sequence (AGGAGT) for translation initiation starting at −11 bp is marked by a wavy line. The amino-terminal amino acid sequence determined from purified PPK2 (underlined) agrees with the deduced amino acid sequence from the start codon. The incorrect original annotation of the start codon at nucleotide 178 (GTG) is shaded. The P-loop sequence is marked by a dotted line, and the conserved amino acids are shaded. Three aspartyl-prolyl (D-P) bonds are identified in boxes.