Fig 5.

p160 corepression at the octeocalcin GRE. (A) GRIP1 and SRC1 potentiate GR-mediated repression of osteocalcin. U2OS.G cells were transfected with a pOS-344-Luc reporter, β-actin-LacZ, and indicated amounts of GRIP1 or SRC1 (equalized with pCDNA3). Reporter activity was assessed in the absence (gray) or presence (black) of Dex as described in Fig. 1. (B) GRIP1 repression domain is dispensable for corepression at the osteocalcin GRE. U2OS.G cells were transfected with indicated amounts of WT (wt) GRIP1 or GRIP1 ΔRD (RD−), and the activities of the pOS-344-Luc (OC) and AP-1-Luc (AP1) reporters were assessed in the absence or presence of Dex and plotted as “fold repression” at each amount of transfected GRIP1 derivative.