Table 1.

Complementation of the E. coli pth^ts mutant with pth and pth2 genes

Plasmids	Gene	Incubation temperature, °C		Cured clones incubated at 42°C
		32	42
pKQV4	None	+	−	ND
pGI01	E. coli pth	+	+	–
pGR518	MJ0051	+	+	–
pGR87L	YBL057c	+	+	ND
pGRES	YHR189w	+	+	ND

E. coli C600 pth^ts was transformed with plasmids containing the indicated genes, plated on LB plates, and incubated at the specified temperature. +, growth; −, no growth; ND, not determined.

^*The genes were cloned into pKQV4. The empty vector was a negative control.

^† The N-terminal 86 amino acids were deleted from the complete ORF.