Table 1.

Cβ distances and solvent-accessible surface of mutated residues in alternative conformations

Mutations	Cβ distance, Å		Residue	Accessibility, %
	Open	Closed		Open	Closed
Locked open
Q163C/Q309C	6.4	7.2	Q163	50	41
			Q309	18	16
D294C/Q311C	7.1	10.0	D294	40	40
			Q311	26	54
F297C/A304C	3.8	10.2	F297	10	8
			A304	0	23
Locked closed
Q163C/R313C	13.3	5.2	Q163	50	41
			R313	86	20

^*Cβ distances were calculated from the open (4) and closed (5) αM I domain crystal structures.

^† Solvent-accessible surface area as percentage of theoretical maximum calculated with joy (28).